Table 1.

Strain list

StrainGenotypeaSource or reference
FY254 h urad4-D18 leu1-32 ade6-M210 can1-1 Our stock
FY440 h + rad4-116 ura4-D18 leu1-32 15
FY818 h +/h Δhsk::ura4 +/hsk1 + ura4-D18/ura4-D18 leu1-32/leu1-32 ade6-M210/ade6-M216 36
FY945 h hsk1-1312 ura4-D18 leu1-32 ade6-M210 This study
FY961 h + mis5-268(mcm6) ura4-D18 leu1-32 ade6-M210 can1-1 M. Yanagida
FY980 h hsk1-1312 mcm5HA::leu1 + leu1-32 ura4-D18 leu1-32 ade6-M216 This study
FY981 h hsk1-1312Δmcm2::(mcm2HA-leu1 +)leu1-32 ura4-D18 leu1-32 ade6-M210 This study
FY982 h hsk1::p(hsk1HA-ura4 +)ura4-D18 leu1-32 ade6-M210 can1-1 This study
FY983 h + hsk1-1312 mis5-268 ura4-D18 leu1-32 ade6-M210 This study
FY984 h + hsk1-1312 cdc19-P1 ura4-D18 leu1-21 ade6-M210 This study
FY998 h hsk1-1312 cdc21-M68 ura4-D18 leu1-32 ade6-M210 This study
FY999 h + hsk1-1312Δcds1::ura4 + ura4-D18 leu1-32 This study
FY1000 h cdc10-V50 hsk1::p(hsk1HA-ura4 +)ura4-D18 leu1-32 ade6-M210 This study
FY1002 h cdc21-M68 hsk1::p(hsk1HA-ura4 +)ura4-D18 leu-32 ade6-M210 This study
FY1011 h cdc22-M45 hsk1::p(hsk1HA-ura4 +)ura4-D18 leu-32 ade6-M210 This study
FY1035 h hsk1::p(hsk1-1312HA-ura4 +)ura4-D18 leu1-32 ade6-M210 can1-1 This study
FY1072 h + hsk1-1312 cdc25-22 ura4-D18 leu1-32 ade6-M216 This study
FY1082 h +Δcds1::ura4 + hsk1::p(hsk1HA-ura4 +)ura4-D18 leu1-32 ade6-M210 This study
FY1085 h +Δchk1::ura4 + hsk1::p(hsk1HA-ura4 +)ura4-D18 leu1-32 ade6-704 This study
FY1089 h + cdc23-M36 ura4-D1 leu1-32 ade6-M210 This study
FY1105 h Δrad3::ura4 + ura4-D18 leu1-32 ade6-704 P. Russell
FY1124 h + hsk1-1312 rad4-116 ura4-D18 leu1-32 This study
FY1125 h + hsk1-1312 cdc23-M36 ura4-D18 leu1-32 ade6-M210 This study
FY1127 mat2-102 hsk1-1312 ura4-D18 leu1-32 ade6-M216 This study
FY1132 h + hsk1-1312 orp1-1 ura4-D18 leu1-32 ade6-M210 This study
FY1135 h hsk1-1312 cdc24-G1 ura4-D18 leu1-32 ade6-M210 This study
FY1137 h + hsk1-1312 pol1-1 ura4-D18 leu1-32 ade6-M210 This study
FY1159 h rad21-K1::ura4 + ura4-D18 leu1-32 ade6-M216 his7-366 57
FY1163 h rad12::ura4 + ura4-D18 leu1-32 ade6-M210 10
FY1176 h + chk1:ep leu1-32 ade6-M216 59
FY1181 h hsk1-1312 chk1::ep ura4-D18 leu1-32 ade6-M210 This study
FY1282 h cdc10-V50 hsk1-1312 ura4 leu1 ade6-M210 This study
HSY1 h /mat2-102 ura4-D18/ + leu1-32/+ ade6-M210/ade6-M216 This study
HSY2 h /mat2-102 hsk1-1312/hsk1-1312 ura4-D18/ura4-D18 leu1-32/leu1-32 ade6-M210/ade6-M216 This study
  • a All strains are isogenic withh 972. We have described previously other strains used in this work (34, 53).