Table 3.

Plasmids used in this study

PlasmidYeast geneMarker(s)Parent vectorReference or source
pRS425Empty vector LEU2, 2μm 5
pRS426Empty vector URA3, 2μm 5
p1873 GCD1 GCD6 LEU2, 2μmpRS425 9
p1871 GCD2 GCD7 GCN3 URA3, 2μmpRS426 9
p2297 GCD2 URA3, 2μmpRS426 29
p2302 GCD1 GCD6 URA3, 2μmpRS426 29
p2304 GCN3 URA3, 2μmpRS426 29
p2305 GCD7 URA3, 2μmpRS426 29
pAV1139 GCD2 GCD7-S119P GCN3 URA3, 2μmpRS426 21
pAV1140 GCD2 GCD7-I118T D178Y GCN3 URA3, 2μmpRS426 21
pAV1089 SUI2 SUI3 GCD11-His URA3, 2μmpRS426 21
p1861 GST Ampr pGEX-5X-3Pharmacia
p2565 GST-SUI2 Ampr pGEX-5X-3W. Yang
pTK29 SUI2 LEU2, 2μmpRS426This study
pTK1.11 GCD1-2xFlag-His GCD6 URA3, 2μmpRS425This study
pTK4 PKR-Flag-His under GAL1-CYC1promoter URA3, leu2-d, 2μmpEMBLyex4This study
pTK6 GST-SUI2-S51A Ampr pGEX-5X-3This study
pTK17 GST-SUI2-His (aaa1–245)Ampr pGEX-6p-2This study
pTK18 GST-SUI2-His (aa 1–197)Ampr pGEX-6p-2This study
pTK19 GST-SUI2-His (aa 1–140)Ampr pGEX-6p-2This study
pTK20 GST-SUI2-His (aa 1–100)Ampr pGEX-6p-2This study
pTK22 GST-SUI2-K79A Ampr pGEX-5X-3This study
pTK23 GST-SUI2-G80A Ampr pGEX-5X-3This study
pTK24 GST-SUI2-D83A Ampr pGEX-5X-3This study
pTK26 GST-SUI2-R88T Ampr pGEX-5X-3This study
pTK27 GST-SUI2-E49N Ampr pGEX-5X-3This study
pTK28 GST-SUI2-E49Q Ampr pGEX-5X-3This study
  • a aa, amino acids.