Table 1.

Subcellular distribution of GFP fusion proteins after TSA treatment

Distribution% of cellsa with fluorescence due to:
Wild-type CIITAmK1mK2p65
−TSA+TSA−TSA+TSA−TSA+TSA−TSA+TSA
N > C33 ± 377 ± 8.50.5 ± 0.20.6 ± 0.20.4 ± 0.22.2 ± 0.713.3 ± 3.114.2 ± 4
N = C54.4 ± 4.219.5 ± 33.5 ± 15.5 ± 214.4 ± 2.760.4 ± 8.114.7 ± 413.1 ± 3.5
C > N12.6 ± 23.5 ± 0.896 ± 1.293.4 ± 385.2 ± 2.837.4 ± 7.272 ± 6.772.7 ± 6.5
  • a The percentage of cells with clearly visible nuclear over cytoplasmic fluorescence (N > C), fluorescence homogeneously distributed in both nucleus and the cytoplasm (N = C), or predominantly cytoplasmic fluorescence (C > N) is shown 24 h posttransfection without (−) or in the presence of (+) 1 μM TSA. The results shown are the average percentages together with their standard deviation derived from five experiments. During each experiment, more than 1,000 cells were scored.