Table 4.

Filament formation on SLAD medium

PlasmidFilament formationa
CG31 (WT)HLY351 (ste7/ste7)HLY352 (ste12/ste12)HLY2002 (tec1/tec1)HLY852 (flo8/flo8)HLY1873b (phd1/phd1 ste12/ste12)
Vector+++ (80)+ (10)+ (10)++ (10)+ (50)
− (20)− (90)− (100)− (90)− (90)− (50)
CRK1 +++ (80)+ (20)+ (10)++ (10)++ (50)
− (20)− (80)− (100)− (90)− (90)+ (50)
CRK1N +++++ (80)+++++ (50)++++ (40)+++++ (50)++ (10)+++++ (50)
− (20)+ (50)+ (60)+ (50)− (90)+ (50)
  • a −, smooth colonies; +, filamentous colonies. The level of cell elongation is indicated by the number of +'s. The percentage of filamentous or smooth colonies in total colonies counted is given in parentheses.