Table 1.

Induction of interchromosomal recombination by a DSB

Cell lineDNARecombination frequency (10−6)a
F5′pCBASce<0.084
pΔnar<0.084
pCBASce + pΔnar260 ± 92
F5′/3′pCBASce3.9 ± 2.3
pΔnar<0.053
pCBASce + pΔnar150 ± 24
R5′/3′pCBASce0.42 ± 0.19
pΔnar<0.048
pCBASce + pΔnar100 ± 12
  • a Recombination frequency was calculated as the total number of G418R clones divided by the total number of cells surviving electroporation and was derived from at least three experiments for each cell line. Two independently derived cell lines for each F5′/3′ and R5′/3′ were tested (Fig. 1).