Table 3.

Effect of pH on the growth of S. cerevisiae at different ammonium concentrations

(NH4+ + NH3) concentration (mM)Doubling time (min) at pH:a
5.56.57.58.0
7165165220420b
701651701,200b NGc
  • a Cells were grown in medium 164 with glucose (3%) as the carbon source and ammonium as the sole nitrogen source. At pH values of 7.5 and 8.0, 50 mM Tris was added to the medium for buffering. The wild-type strain was 23344c.

  • b Initial doubling time.

  • c NG, no growth.