Table 2.

Growth of S. cerevisiae at pH 6.1 on different nitrogen sources

Nitrogen sourceaConcentration (mM) of nitrogen sourceDoubling time (min)
Wild typebΔmep1 Δmep2Δmep3b Δvph1
Glutamate10160160230
Proline10200200410
Arginine2.5160170180
Urea5270270450
NH4Cl20160175205
5160290210
1175>700215
  • a Cells were grown in medium 164 with glucose (3%) as the carbon source and different nitrogen sources at the concentrations indicated. Strains were 23344c (wild type), 31019b (Δmep1 Δmep2 Δmep3) (35), and NCM3243 (Δvph1).

  • b Final ODs of strains 23344c and 31019b were 13 and 13 on glutamate, 12 and 13 on proline, 11.5 and 11.5 on arginine, 14 and 13.5 on urea, 16 and 16 on 20 mM NH4Cl, 10 and 7 on 5 mM NH4Cl, and 1.3 and 0.4 on 1 mM NH4Cl.