Table 3.

Computational search of potential Cph2 and Tec1 binding sitesa

GeneCph2 sitesTec1 sites (AbaA)
SRE-1E-box
ECE1 GTCACCTCAC (−724, −715)TATCACCTGAT (−316, −306)TCATTCT (−371, −365)
HWP1 TATCACCTGTA (−222, −212)TCATTCT (−153, −147)
HYR1 TCATTCT (−513, −519), TCATTCC (−270, −264), TCATTCT (−32, −26)
RBT4 GTCATACCAC (−618, −609)GCATTCT (−532, −526)
SAP5 TCATTCC (−116, −110), TCATTCC (−91, −85), TCATTCC (−66, −60)
ALS3 TCATTCT (−808, −802)
Total no. of potential sites2210
Expected random occurrence0.356 × 6 = 2.140.235 × 6 = 1.410.728 × 6 = 4.37
  • a SRE-1 and E-box weight matrices (see Fig. 6A) and the Tec1 consensus sequence were used to search for potential Cph2 and Tec1 binding sites in the upstream sequences of hypha-specific genes. The sequences and positions of putative binding sites found from the search are shown. The expected random occurrence of a motif in this set of hypha-specific genes is calculated by multiplying the frequency of its occurrence in the control set by the number of hypha-specific genes.