Table 2.

Deletion of as few as 24 amino acids of Sir3p abolishes the interaction with Rap1p but not Sir3p or Sir4p in the two-hybrid system

GAD/Sir3p hybridβ-Galactosidase (U)
LexA/Rap1p (679–827)LexA/Sir4p (839–1358)LexA/Sir3p (307–978)
GAD/Sir3p(307–978)5,8243,2383,597
GAD/Sir3p(Δ456–479)34,2971,384
GAD/Sir3p(Δ440–480)43,3541,201
GAD/Sir3p(Δ440–502)43,3311,704
GAD/Sir3p(Δ440–454)5,3373,5553,292
GAD/Sir3p(Δ398–437)5,7092,9443,136
GAD/Sir3p(Δ358–437)6,5872,5013,333
GAD/Sir3p(Δ333–357)6,4452,8453,378
GAD/Sir3p(Δ482–502)7,0522,3292,694
GAD 352