TABLE 5

Guide RNAs used in this study

PrimerTargeting typeForward sequenceReverse sequence
ControlNot applicable5′-GTCCCCTCCACCCCACAGTG-3′5′-CACTGTGGGGTGGAGGGGAC-3′
gRNA set 1Promoter + exon5′-AGAAGGAGATCACTGCCCTGGCACC-3′5′-CCTGCTTGCTGATCCACATCTGCTG-3′
gRNA set 2Promoter + exon5′-AGAAGGAGATCACTGCCCTGGCACC-3′5′-TTATTTCGGAGCGAGAGCCG-3′
gRNA set 3Promoter + exon5′-CTCCCCGCTCCTCTCCGCGA-3′5′-TTATTTCGGAGCGAGAGCCG-3′