Table 1.

Quantitative immunoblotting analysis of various Lyn constructs and endogenous Lyn and Src kinase in lipid raft fractions after solubilization of transfected RBL cells with different concentrations of Triton X-100a

Kinase analyzedMean % of kinase in lipid raft fraction ± SD (no. of expt) at Triton X-100 concn:
0.06%0.1%0.2%
Transfected kinase
 LynA-WT31.1 ± 2.7 (3)30.4 ± 3.2 (3)28.2 ± 4.0 (3)
 LynB-CA5.3 ± 1.0 (4)0.0 ± 0.0 (2)0.0 ± 0.0 (2)
 LynB-GCA0.0 ± 0.0 (2)NTNT
Endogenous kinase
 Lyn54.9 ± 5.9 (7)51.2 ± 1.9 (3)47.2 ± 5.2 (3)
 Src18.8 ± 3.3 (4)14.4 ± 2.0 (3)0.0 ± 0.0 (2)
  • a Values indicate means ± SD of the percentage of kinases in lipid raft fractions (15 to 30% sucrose) after solubilization of the cells in the indicated concentrations of Triton X-100 and fractionation on sucrose density gradients. Numbers in parentheses indicate number of independent experiments performed. NT, not tested.