TABLE 2.

H1 subtype composition and total H1 stoichiometry per nucleosome in wild-type and H1 compound-knockout micea

Tissue and genotype% of total H1bTotal H1/nucleosomec (no. of determinations)
H1°H1aH1bH1cH1dH1e
Thymus
    Wild type (+/+)1.9 ± 1.610.7 ± 1.316.5 ± 0.926.7 ± 3.535.4 ± 2.79.6 ± 1.50.83 ± 0.10 (12)
    H1°−/−; H1cH1e−−/−−017.0 ± 3.528.3 ± 3.0054.7 ± 5.500.47 ± 0.26 (8)
    H1cH1dH1e−+−/−−−5.9 ± 4.625.0 ± 3.035.4 ± 3.1031.7 ± 2.600.41 ± 0.16 (6)
Liver
    Adult
        Wild type (+/+)29.0 ± 0.81.4 ± 0.615.8 ± 3.512.5 ± 1.014.3 ± 0.639.9 ± 2.50.79 ± 0.07 (5)
        H1°−/−; H1cH1e−−/−−02.2 ± 0.36.3 ± 1.3091.5 ± 1.600.64 ± 0.01 (4)
    Neonatal
        Wild type (+/+)9.5 ± 4.96.1 ± 2.414.2 ± 1.720.9 ± 4.530.3 ± 2.319.0 ± 1.50.76 ± 0.07 (5)
        H1cH1dH1c−+−/−−−26.7 ± 6.59.9 ± 2.229.3 ± 4.6032.7 ± 5.200.50 ± 0.15 (6)
  • a Data are from HPLC analyses of wild-type and H1 compound-knockout mice. Values are means ± standard deviations of individual determinations made on each of the indicated genotypes.

  • b Determined by ratio of A214 of the indicated H1 peak to the total A214 of all H1 peaks. The A214 values of the individual H1 peaks were adjusted to account for the differences in the number of peptide bonds in each H1 subtype.

  • c Determined by the ratio of the total A214 of all H1 peaks to half of the A214 of the H2b peak. The A214 values of the H1 and H2b peaks were adjusted to account for the differences in the number of peptide bonds in each H1 subtype and H2b.