TABLE 1.

Numbers of transformants obtained with active HOG1 alleles (under the control of a strong constitutive promoter) and their ability to support growth under osmotic pressure

HOG1 allelehog1Δ cellspbs2Δ cells
Approx no. of colonies/μg of DNAGrowth on 1.3 M NaClbApprox no. of colonies/μg of DNAGrowth on 1.3 M NaClb
Y68H,A314TA few thousand+A few thousand+
Y68H,F318LA few thousand+A few thousand+
Y68H,F318SA few thousand+A few thousand+
Y68H,W320RA few thousand+A few thousand+
Y68H,F322LA few thousand+A few thousand+
Y68H,W332LA few thousand+A few thousand
D170A,A314THundreds of tiny colonies+Hundreds of tiny colonies+
D170A,F318LNo coloniesaHundreds of tiny colonies+
D170A,F318SNo coloniesaNo coloniesa
D170A,W320RHundreds of tiny coloniesHundreds of tiny colonies+
D170A,F322LHundreds of tiny coloniesHundreds of tiny colonies+
D170A,W332LHundreds of tiny coloniesHundreds of tiny colonies+
  • a In these transformations, 30 to 70 big colonies appeared and were considered a consequence of rearrangement events. Some of these colonies were tested on salt and were found negative.

  • b +, growth observed; −, no growth observed.