TABLE 2.

Targeted integration frequency in wild-type and fancg mutant cells

Targeting constructTargeted integration frequencya
Wild typefancgComplemented with GdFANCG
Clone 1Clone 2Clone 3
KU70-His14/34 (41) [2.1 × 10−6]10/46 (22) [2.0 × 10−6]NDbND22/36 (61) [3.8 × 10−6]
XRCC2-Bsr16/37 (43) [2.0 × 10−6]ND6/24 (25) [7.9 × 10−6]6/24 (25) [3.8 × 10−6]18/28 (64) [4.8 × 10−6]
XRCC3-His8/25 (32) [8.3 × 10−7]1/22 (4.5) [7.3 × 10−7]NDND9/20 (45) [6.6 × 10−7]
Ovalbumin-Bsr24/26 (92) [3.9 × 10−5]24/28 (86) [3.8 × 10−5]NDNDND
  • a Data are numbers of targeted clones at each locus per number of drug resistant clones analyzed by Southern blotting. The percentage of targeted integration events is given in parentheses. Numbers in brackets indicate transfection efficiency. Results for three different fancg mutant clones and one clone complemented with chicken FANCG cDNA are shown.

  • b ND, not determined.