TABLE 1.

ΔNp73 cooperates with oncogenic Ras in promoting tumorigenicity in vivo

MEF typeNo. of mice with tumors/ total no. of miceNo. of tumors/mouseAvg wt of tumor (g)Mean latency period (wk) ± SD
129 MEFsa
    GFP+K-RasV120/4NAeNANA
    ΔNp73+K-RasV123/51, 1, 21.7510 ± 3
    ΔNp73+N-RasD122/21, 20.6210 ± 3
    ΔNp73+H-RasV122/41, 20.534 ± 1
    p53 H175+K-RasV125/52, 2, 2, 2, 21.372.5
    p53 H175+N-RasD121/121.53
B6 MEFsb
    GFP+H-RasV120/4NANANA
    ΔNp73+H-RasV122/41, 10.259 ± 2
    ΔNp73+K-RasV120/4NANANA
    p53−/−+H-RasV122/22, 20.62.5
    p53 H175+H-RasV122/22, 20.62.5
Spontaneously immortalized ΔNp73 129 MEFsc
    +H-RasV12 (expt 1)2/22, 20.451.4d
    +H-RasV12 (expt 2)2/22, 20.521.4d
Passaged and in vitro-transformed 129 MEFs (ΔNp73+H-RasV12)2/21, 10.74.5 ± 1
p73−/− MEFs (GFP+H-RasV12)0/2NANANA
  • a MSCV based.

  • b Percent Rpuro based, after selection.

  • c Expt 1, two populations pooled, MSCV based; expt 2, three clones pooled, Rpuro based after selection.

  • d That is, 10 days.

  • e NA, not applicable.