TABLE 1.

Induction of UPR target genes in wt, XBP-1−/−, and iATF6α MEF cells by Tm treatment and in MEF-tet-off-XBP-1s cells by doxycycline removal

Fold change in expression with:GenBank no.Description
wtXBP-1−/−iATF6αXBP-1s
27.116.78.5NCX67083CHOP
21.47.127.2NCU00937GADD45
117.24.43.2AI846938Herp
4.6NC2.21.9AI845538MGP
8.475.5NCAW045664RIKEN 2810026P18, GADD7
4.52.82.81.7AJ002387BiP
4.33.32.2NCAA684508Rnu22, RNA, U22 small nucleolar
2.93.32.6NCX143094F2 antigen
2.8NC1.6NCAA260005Par-4
9.85.89.1NCU19118LRG-21
4.82.24NCAI840585RIKEN 3110043O21
4.53.23.7NCL00039c-myc
3.63.12.62.7AW122364Armet
2.93.43NCAB0171894F2/CD98
2.83.22.6NCAI849615Gas5, growth arrest specific 5
2.91.81.63.1U28423p581PK
8.92.47.95AW120711ERdj4
8.8∼15.04.92AW124049EST, Genethonin
5.23.71.9NCAI852641Nupr1, nuclear protein 1
4NC2.92.2AW123880XBP-1
3.93.23.7NCAI854851RIKEN 2700007P21
3.835.5NCU52073TDD5, androgen target gene
3.45.23.8NCU40930Sqstm1, squestosome
3.3NC1.82.7AI604013p581PK
3.33.12.9NCU83148NFIL3/E4BP4
2.92.32.41.5V00756Beta interferon
2.82.62.6NCU13371Kdt1, kidney cell line derived transcript I
2.72.6NCNCU79550Slug, chicken homolog
2.52.61.8NCM94087ATF4
2.4NCNCNCAV138783Gadd45b
9.6NC9.25.7AI835630ERdj4
4.63.86.1NCU60593Ndr1
4.2NC3.7NCAA798624Ero11
4.25.36.1NCM31418Interferon activated gene 202
4.13.64.7NCAI839690RIKEN 1500005G05
3.7NC2.7NCM95200Vascular endothelial growth factor
2.73.12NCJ04627Methenyltetrahydrofolate cyclohydrolase
2.7NC2.2NCAW047899Nfkb2
2.42.21.9NCX78709NRF1
2.22.12.3NCAI849620EST
2NCNCNCJ03297grp94
  • a Values represent fold changes of expression level by Tm treatment for 6 h in wt, XBP-1−/−1, and iATF6α MEF cells by Tm or doxycycline removal in MEF-tet-off-XBP-1s cells. NC, no change. Genes were sorted by ratio of oligonucleotide probe pairs on the chip whose values were increased upon Tm treatment in wt cells, and the genes whose values were >0.8 are shown. The two XBP-1-dependent genes which were confirmed by Northern blot analysis are highlighted in boldface.