TABLE 3.

Half-life of HA-Nrf2a

Keap1 proteinbTreatmentct1/2 (h)d
None2.7
None+3.2
WT0.6
WT+2.5
C151S0.6
C151S+0.6
C273S6.5
  • a HA-Nrf2 was expressed in MDA-MB-231 cells in the absence or presence of Keap1 proteins and in the absence or presence of sulforaphane.

  • b The indicated Keap1 proteins (Fig. 1B) were coexpressed in MDA-MB-231 cells with HA-Nrf2. WT, wild type.

  • c Transfected MDA-MB-231 cells were treated with DMSO (−) or 4 μM sulforaphane (+) for 16 h prior to and during the pulse-chase experiment.

  • d The half-life of HA-Nrf2 was determined by pulse-chase labeling.