TABLE 1.

Response of C. albicans strains to α-factora

StrainCell typeGenotypeTreatmentNo. of cells with projections/total no. of cells% Cells with projections
CHY257α whiteΔa 11α2 10% DMSO0/220<0.5
CHY420 a white a 1/Δα1Δα2 10% DMSO0/225<0.5
RBY731 a/a white MTL a/MTL a 10% DMSO2/2360.8
RBY734α/α white MTLα/MTLα10% DMSO1/2190.4
CHY257α whiteΔa 11α2 α-Factor0/246<0.5
CHY420 a white a 1/Δα1Δα2 α-Factor0/240<0.5
RBY731 a/a white MTL a/MTL a α-Factor3/2351.3
RBY734α/α white MTLα/MTLαα-Factor1/2270.4
CHY257α opaqueΔa 11α2 10% DMSO0/227<0.5
CHY420 a opaque a 1/Δα1Δα2 10% DMSO2/2330.8
RBY731 a/a opaque MTL a/MTL a 10% DMSO1/2210.4
RBY734α/α opaque MTLα/MTLα10% DMSO0/219<0.5
CHY257α opaqueΔa 11α2 α-Factor5/2172.3
CHY420 a opaque a 1/Δα1Δα2 α-Factor41/21519
RBY731 a/a opaque MTL a/MTL a α-Factor121/21556
RBY734α/α opaque MTLα/MTLαα-Factor1/2210.4
  • a α-Factor in 10% DMSO was added at a concentration of 10 μg/ml. Cells were scored for formation of elongated projections 4 h after DMSO or α-factor was added to the cells. This experiment shows that to respond efficiently to α-factor, cells must be of mating type a and have been switched to the opaque phase.