TABLE 2.

Mating efficiency of mfα and ste2 mutants of C. albicans a

Strain (Ura)Mating typeRelevant genotypeMating efficiency
MMY278 a (Ade)CHY477 α (Ade)
CHY439 a Wild type<10−8 2.3 × 10−1
CHY257αWild type4.8 × 10−1 3.6 × 10−7
MMY564 a mfα/mfα<10−8 5.0 × 10−2
MMY563α mfα/mfα<10−8 <10−8
MMY566 a ste2/ste2 <10−8 <10−8
MMY565α ste2/ste2 1.8 × 10−1 <10−8
MMY567 a ste2/ste2 <10−8 <10−8
MMY568α ste2/ste2 1.6 × 10−1 5.4 × 10−8
  • a Gene knockouts of MFα and STE2 were created, and opaque a and α derivatives (Ura) mated with opaque control mating strains (Ade). The frequency of mating was determined by the number of colonies that grew on Ura Ade medium, as described in Materials and Methods.