TABLE 2.

Subcellular distribution of HA-Nrf2a

Keap1 proteinb% Subcellular distribution inc:
NN-CC
Noned01000
WT00100
ΔN00100
ΔBTB00100
ΔL50500
ΔK01000
ΔC00100
  • a The subcellular localization of HA-Nrf2 in NIH 3T3 cells was determined by indirect immunofluoresence.

  • b The indicated Keap1 proteins were coexpressed with Nrf2 in NIH 3T3 cells. WT, wild type.

  • c Percentages of cells that displayed predominantly nuclear (N), nuclear/cytoplasmic (N-C), and cytoplasmic (C) staining of Nrf2 are presented. A total of 100 cells that are positive for expression of Nrf2 and the indicated Keap1 protein were examined.

  • d HA-Nrf2 was expressed alone (without a Keap1 protein) in NIH 3T3 cells.