TABLE 1.

Summary of methylation status, expression levels of GATA genes, and candidate target genes in cancer cells before and after DAC treatment

GeneRKODLD1HCT116HT29LoVoSW480AZ521Normal colonPBLc
MtaExprbMtExprMtExprMtExprMtExprMtExprMtExprMtExprMtExpr
MAMAMAMAMAMAMA
GATA-4U++M+M+M+U/M+++M+M+U+U
GATA-5M+M+M+M+U/M+++M+M+U+U
GATA-6U++U++U++U++U++U++U++U+U+
TFF1M+U+++M+U+++U+++M+M+U/M+M
TFF2NAND++M+M++ND++ND++NAND+ND
TFF3ND+ND++M+M++ND+++ND++ND+ND+ND
inhibinαU++M+M+U/M+/−U/M+++U+++U+++UU
Dab2M+U++U++U++U++U++U++U+U+
  • a Mt, methylation status. Abbreviations: U, unmethylated; M, methylated; NA; genomic sequences were not dected by either MSP and bisulfite sequencing; ND; not done.

  • b Expr, expression. Abbreviations: M, mock treatment; A, DAC treatment. Key: −, undetectable level; +, detectable level; ++, increased expression level from the “+” basal level. Analyses were done by RT-PCR.

  • c PBL, peripheral blood lymphocytes.