TABLE 2.

Plasmids used in this study

PlasmidMarkersSource or reference
YEp3512μm LEU212
pS3242μm LEU2 YEp13 + IME2Saul Honigberg
pMR12μm URA3 pRS426 + IME227
pAM4pMR1 + 13mycThis study
pSM1pAM4 + ime2-Y244F-mycThis study
pSM2pAM4 + ime2-T242A, Y244F-mycThis study
pSM3pAM4 + ime2-S246A-mycThis study
pSM6pAM4 + ime2-Y241F-mycThis study
pSM10pAM4 + ime2-T242A-mycThis study