TABLE 1.

Quantitative analysis of immunoprecipitation of cross-linked chromatin of CarX and CarX-TDR transgenes

AntibodyMethodaSignal intensity ratiob
CarX (n)CarX-TDR (n)
Anti-GAGA factor antibodies11.24 ± 0.33 (16)2.24 ± 0.55 (16)
20.84 ± 0.14 (5)1.26 ± 0.17 (5)
Anti-TBP antibodies11.99 ± 0.27 (16)1.12 ± 0.14 (16)
21.27 ± 0.07 (5)0.78 ± 0.12 (5)
  • a Method 1, DraI digestion of the long PCR product; method 2, a second round of PCR amplification (see the legend to Fig. 4 and Materials and Methods).

  • b Mean ± standard error of the mean of the ratio of intensity of signal with the fragment of transgene to the fragment of endogenous gene in preparations treated by antibodies and normalized to the same ratio from the mock experiment (see Materials and Methods). The number of experiments for each transgene (n) is shown in parentheses.