TABLE 1.

S. cerevisiae strains used in this studya

StrainGenotypeSource and/or reference
JHUY669MATa est1Δ::LEU2 his3Δ1::pJHU618 ade2Δ::hisG can1Δ::MET15 ura3Δ0 leu2Δ0 lys2Δ0 met15Δ0; VII-L, CAN1; VII-R, URA3
YPH450MATα ade2 can1 ura3 leu2 his7 trp1; VII-L, ade5 lys5 cyh2 trp5 leu1Derived from A364A (7)
JHUY676MATa/MATα est1Δ::LEU2/EST1 his3Δ1::pJHU618/HIS3 ade2Δ::hisGlade2 can1Δ::MET15/can1 ura3Δ0/ura3 leu2Δ0/leu2 lys2Δ0/LYS2 met15Δ0/MET15 HIS7/his7 TRP1/trp1; VII-L, (ADE5 CAN1 LYS5 CYH2 TRP5 LEU1)/(ade5 lys5 cyh2 trp5 leu1); VII-R, URA3/( ); pJHU840JHUY669 × YPH450 + pJHU840
JHUY677MATa/MATα est1Δ::LEU2/est1Δ::kanMX his3Δ1::pJHU618/HIS3 ade2Δ::hisGlade2 can1Δ::MET15/can1 ura3Δ0/ura3 leu2Δ0/leu2 lys2Δ0/LYS2 met15Δ0/MET15 HIS7/his7 TRP1/trp1 VII-L, (ADE5 CAN1 LYS5 CYH2 TRP5 LEU1)/(ade5 lys5 cyh2 trp5 leu1); VII-R, URA3/(); pJHU840est1Δ/est1ΔJHUY676
JHUY704MATa cdc13-1 his3Δ1::pJHU618 ade2Δ::hisG can1Δ::hphMX ura3Δ0 lys2Δ0 met15Δ0; VII-L, CAN1; VII-R, kanMX
JHUY671MATα cdc13-1 ade2 can1 ura3 leu2 his7 trp1; VII-L, ade5 lys5 cyh2 trp5 leu1
JHUY705MATa/MATα cdc13-1/cdc13-1 his3Δ1::pJHU618/HIS3 ade2Δ::hisG/ade2 can1Δ::hphMX/can1 ura3Δ0/ura3 LEU2/leu2 lys2Δ0/LYS2 met15Δ0/MET15 HIS7/his7 TRP1/trp1; VII-L, (ADE5 CAN1 LYS5 CYH2 TRP5 LEU1)/(ade5 lys5 cyh2 trp5 leu1); VII-R, kanMX/()JHUY704 × JHUY671
JHUY707MATa his3Δ1::pJHU618 ade2Δ::hisG can1Δ::hphMX ura3Δ0 lys2Δ0 met15Δ0; V-L, ARS121 3A-loxP; VII-L, UR-loxP CAN1; VII-R, kanMX
JHUY706MATα ade2 can1 ura3 leu2 met15Δ0 his7 trp1; VII-L, ade5 lys5 cyh2 trp5 leu1Deletion of MET15 with pAD4 (5) in YPH450
JHUY708MATa/MATα his3Δ1::pJHU618/HIS3 ade2Δ::hisGlade2 can1Δ::hphMX1can1 ura3Δ0/ura3 LEU2/leu2 lys2Δ0/LYS2 met15Δ0/met15Δ0 HIS7/his7 TRP1/trp1; V-L, (ARS121 3A-loxP)/(); VII-L, (UR-loxP ADE5 CAN1 LYS5 CYH2 TRP5 LEU1)/(ade5 lys5 cyh2 trp5 leu1); VII-R, kanMX/()JHUY707 × JHUY706
JHUY709MATa/MATα est1Δ::LEU2/EST1 mre11Δ::hphMX/MRE11 yku70Δ::kanMX/YKU70 his3Δ1::pJHU618/his3Δ1::pJHU618 ade2Δ::hisGlade2Δ::hisG can1Δ::MET15/can1Δ::MET15 ura3Δ0/ura3Δ0 leu2Δ0/leu2Δ0 lys2Δ0/LYS2 met15Δ0/met15Δ0; XV-L, (ADE2-CAN1 URA3)/(ADE2-CAN1 URA3) (TELO)Derived from JHUY635 (27)
JHUY710MATa/MATα est1Δ::LEU2/EST1 exo1Δ::kanMX/EXO1 his3Δ1::pJHU618/his3Δ1::pJHU618 ade2Δ::hisGlade2Δ::hisG can1Δ::MET15/can1Δ::MET15 ura3Δ0/ura3Δ0 leu2Δ0/leu2Δ0 lys2Δ0/LYS2 met15Δ0/met15Δ0; XV-L, (ADE2-CAN1 URA3)/(ADE2-CAN1 URA3) (TELO)Derived from JHUY635 (27)
JHUY711MATa/MATα est1Δ::LEU2/EST1 mre11Δ::hphMX/MRE11 exo1Δ::kanMX/EXO1 his3Δ1::pJHU618/his3Δ1::pJHU618 ade2Δ::hisGlade2Δ::hisG can1Δ::MET15/can1Δ::MET15 ura3Δ0/ura3Δ0 leu2Δ0/leu2Δ0 lys2Δ0/LYS2 met15Δ0/met15Δ0; XV-L, (ADE2-CAN1 URA3)/(ADE2-CAN1 URA3) (TELO)Derived from JHUY635 (27)
BY4741MATa his3Δ1 ura3Δ0 leu2Δ0 met15Δ05
JHUY728MATa cdc13-1 his3Δ1 ura3Δ0 leu2Δ0 met15Δ0
JHUY729MATa/MATα est1Δ::LEU2/EST1 rad1Δ::kanMX/RAD1 his3Δ1::pJHU618/his3Δ1::pJHU618 ade2Δ::hisG/ade2Δ::hisG can1Δ::MET15/can1Δ::MET15 ura3Δ0/ura3Δ0 leu2Δ0/leu2Δ0 lys2Δ0/LYS2 met15Δ0/met15Δ0; XV-L, (ADE2-CAN1 URA3)/(ADE2-CAN URA3) (TELO)Derived from JHUY635 (27)
  • a Genotypes on specific chromosome arms are listed from telomere to centromere. Empty parentheses indicate no gene was inserted on the chromosome arm.