TABLE 3.

Structural abnormalities in the flagella of wild-type and Spag6−/− sperma

Sperm typeGenotype% Normal flagella (mean ± SE) for:% Flagella lacking central pair (mean ± SE) for:% Flagella with FS and/or ODF alterations (mean ± SE) for:
Spag6+/+Spag6−/−Spag6+/+Spag6−/−Spag6+/+Spag6−/−
Epididymal spermMP98 ± 240 ± 152.3 ± 232 ± 11*028 ± 4
PP99 ± 168 ± 1208 ± 6*1 ± 224.3 ± 3
Testicular spermMP10077 ± 1100023 ± 11
PP10086 ± 300014 ± 3
  • a Transverse sections of flagella from wild-type and Spag6−/− sperm were examined by transmission electron microscopy. Each cross-section was scored for flagellar abnormalities (absence of central pair of microtubules or alterations in the fibrous sheath and/or outer dense fibers). Sperm from three wild-type and three Spag6−/− mice was examined. A total of 264 Spag6+/+ and 179 Spag6−/− epididymal sperm and 247 Spag6+/+ and 166 Spag6−/− testicular sperm were examined. *, P < 0.001, chi-square test. MP, midpiece; PP, principal piece; FS, fibrous sheath; and ODF, outer dense fibers.