TABLE 2.

Quantitative analysis of immunoprecipitation of cross-linked chromatin of CarX, CarX-mCTd-ΔCT, and CarX-mCTd-ΔCT-ΔTA transgenes

AntibodyMethodaSignal intensity ratiob
CarX (n)CarX-mCTd-ΔCT (n)CarX-mCTd-ΔCT-ΔTA (n)
Anti-GAGA factor antibodies11.21 ± 0.14 (9)0.57 ± 0.12 (6)0.49 ± 0.06 (9)
Anti-TBP antibodies11.36 ± 0.10 (9)0.91 ± 0.19 (8)0.90 ± 0.23 (8)
  • a Method 1, DraI digestion of the long PCR product (see the legend to Fig. 4 and Materials and Methods).

  • b See Table 1, footnote b.