TABLE 1.

Yeast strains and plasmids used

Strain or plasmidGenotype and commentsReference or source
Strains
    NOY408-1af MAT a ade2-1 ura3-1 his3-11 trp1-1 leu2-3,112 rpa135::LEU2 can1-100 fob1::HIS3 pNOY102 pNOY117 27
    NOY408-1b MAT a ade2-1 ura3-1 his3-11 trp1-1 leu2-3,112 can1-100 39
    NOY408-1bfSame as NOY408-1b except fob1::HIS3 27
    TAK899Same as NOY408-1bf except rDNA::URA3
    YK9 MAT a ura3-52 trp1-289 leu2-3-112 prb pep4::TRP1 33
Plasmids
    pNOY102Multicopy plasmid vector; URA3 GAL7-35S rDNA 2μm 39
    pNOY117 CEN6 ARSH4 TRP1 RPA135 Nomura lab
    pEG(KT)Multicopy plasmid vector; URA3 Leu2-d GAL1-GST 2μm 38
    pTAK900pEG(KT) carrying FOB1
    pESC-LEUMulticopy plasmid vector; LEU2 GAL1-FLAG 2μmStratagene
    pTAK901pESC-LEU carrying FOB1
    pTAK991Same as pTAK901 except the ORF of FOB1 has a point mutation (H159A)
    pTAK992Same as pTAK901 except the ORF of FOB1 has a point mutation (H164A)
    pTAK993Same as pTAK901 except the ORF of FOB1 has a point mutation (C193A)
    pTAK994Same as pTAK901 except the ORF of FOB1 has a point mutation (C196A)
    pTAK995Same as pTAK901 except the ORF of FOB1 has a point mutation (D291A)
    YEp24Multicopy plasmid vector; URA3 2μm pBR322 Ampr Tcr New England Biolabs
    pTAK902.1 to -9YEp24 carrying various fragments (1 to 9 in Fig. 3B) around the RFB
    pTAKc1 to -f4YEp24 carrying subfragments (24 bp, c1 to f4 in Fig. 5C) of the RFB
    Yeplac195Multicopy plasmid vector; URA3 2μm 12
    Yep-FOB1Yeplac195 carrying FOB1 27