TABLE 1.

Analysis by subcellular fractionation of the selective activation of endosome-associated EGFR

Cell type and markerYield (%) of indicated fractiona for:
Control cellsCells treated with AG1478 + EGF + monensin
Without EGFWith EGFWithout washWith wash
CYPMENCYPMENCYPMENCYPMEN
BT20
    EGFR0782202872099101090
    p-EGFR000027730000298
    EEA12986331780235634294
MDCK
    EGFR09455158004952296
    p-EGFR00009910000397
    EEA11278151481319783691
  • a Data are the quantification of the results from Fig. 2A normalized against the total yield of the three fractions. Each value is the percentage of the total of each set of three fractions following normalization.