Ability of Q34 cells to form anchorage-independent coloniesa

Cell strain and/or virus% Cells forming agar colonies
Q34 + pBABE4
Q34 + RAS29
Q34 + hTERT + pBABE1
Q34 + hTERT + RAS26
Hs68 + pBABE1
Hs68 + RAS0
Hs68 + hTERT + pBABE0
Hs68 + hTERT + RAS0
  • a Cells were suspended in medium containing 0.2% agarose, and colonies were counted after 21 days. Multiple fields of >100 cells were observed microscopically, and the number of multicellular colonies was estimated as a percentage of the total number of cells visualized.