TABLE 1.

Effects of RNAi of runa

Amt of dsRNA injected (ng/μl)Time after injection (h)Total no. of eggs collectedNo. (%) of dead embryos (%)No. (%) with larval defectRNAi effect rate (%)
0 (TE control)0–121217 (5.8)6 (5.0)13 (10.8)
12–243424 (1.2)5 (1.5)9 (2.6)
24–363311 (0.3)0 (0.0)1 (0.3)
36–482921 (0.3)2 (0.7)3 (1.0)
48–60250 (0.0)0 (0.0)0 (0.0)
200–1230140 (13.3)98 (32.6)138 (45.8)
12–2497357 (5.9)226 (23.2)283 (29.1)
24–361,20432 (2.7)180 (15.0)212 (17.6)
36–481,20956 (4.6)247 (20.4)303 (25.1)
48–605391 (0.2)85 (15.7)86 (16.0)
500–1214215 (10.6)40 (28.2)55 (38.7)
12–2431313 (4.2)60 (19.2)73 (23.3)
24–3637124 (6.5)79 (21.3)103 (27.7)
36–4840129 (7.2)75 (18.7)104 (25.9)
48–6024117 (7.1)38 (15.8)55 (22.8)
  • a The numbers of dead embryos and abnormal larvae were compared to the total numbers of embryos laid at each time interval. Negative control was performed by microinjecting TE alone.