TABLE 1.

 Yeast strains used in this study

StrainaGenotype
RKY1293MATα ura3-52 trp1 leu2Δ1 his3Δ200 pep4::HIS3 prb1Δ1.6R can1 GAL
RKY4220MATa leu2::hisG ho::LYS2 lys2 can1 URA3 HOM3 TRP2 MER3
MATα leu2::hisG ho::LYS2 lys2 CAN1 ura3 hom3-10 trp2 mer3::hisG
RKY4221RKY4220, except mer3::hisG
mer3::hisG
RKY4222RKY4220, except mer3GD
mer3::hisG
RKY4223RKY4220, except mer3KA
mer3::hisG
RKY4227RKY4220, except MER3-FLAG
mer3::hisG
TNY821MATa leu2::hisG ho::LYS2 lys2 his4X::LEU2-URA3 arg4-bgl     MER3
MATα leu2::hisG ho::LYS2 lys2 his4B::LEU2 arg4-nsp mer3::hisG
TNY822TNY821, except mer3::hisG
mer3::hisG
TNY823TNY821, except mer3GD
mer3::hisG
TNY824TNY821, except mer3KA
mer3::hisG
  • a RKY1293 was obtained from the Yeast Genetic Stock Center, Berkeley, Calif., where it is identified as BJ5464. RKY4220, RKY4221, RKY4222, RKY4223, and RKY4227 are congenic with SK1. RKY4221 is identical to TNY368 (41). TNY821, TNY822, TNY823, and TNY824 are derived from SK1.