TABLE 4.

 Analysis of NPD events

Relevant genotypeIntervalResult ata:
30°C23°C
No. of NPDsTotal no. of tetradsProbabilityNo. of NPDs Total no. of tetradsProbability
ObservedExpectedObservedExpected
MER3 CAN1-URA3 15455337.7 × 10−6 17465371.9 × 10−5
URA3-HOM3 11425341.7 × 10−6 18515383.8 × 10−6
mer3GD CAN1-URA3 9155360.123125209.4 × 10−3
URA3-HOM3 5115420.07385210.08
mer3KA CAN1-URA3 5135460.0310105241.00
URA3-HOM3 5105400.11365260.22
  • a Tetrads obtained in Table 2 were examined. The number of NPDs expected was calculated according to the Papazian equation (41a): NPD = 1/2[1 − TT′ − (1 − 1.5TT′)2/3], where TT′ is the frequency of TT shown in Table 2. Chi-square tests were performed to express the likelihood that the difference between the observed and expected numbers was attributable to chance.