TABLE 2.

Plasmids used in this study

PlasmidDescriptionaReference
YCplac33CEN URA3Gietz and Sugino (13)
YEplac1952μm URA3Gietz and Sugino (13)
YEplac1812μm LEU2Gietz and Sugino (13)
pGAD4242μm LEU2 GAL4 (aa 768-881)Fields and Song (10)
pGBT92μm TRP1 GAL4 (aa 1-147)Fields and Song (10)
JK16212μm URA3 4lexA-CYC1-lacZKeleher et al. (20)
pLG132ΔS2μm URA3 CYC1-lacZGuarente and Hoar (18)
pMAL2cMBP expression vectorNew England Biolabs
pRD56GAL1-GST CEN URA3Mitchell et al. (32)
pSLFΔ-178K2μm URA3 CYC1-lacZForsburg and Guarente (11)
pWS412μm HIS3 lexA-SFL1Song and Carlson (43)
pXP3TPK2 in YEplac195Pan and Heitman (35)
pXP23TPK1 in pGBT9 (EcoRI/BamHI)This study
pXP25TPK2 in pGBT9 (BamHI/SalI)This study
pXP26BCY1 in pGAD424 (EcoRI/BamHI)This study
pXP28FLO8 (aa 206-799) in pGAD424 (BamHI/PstI)This study
pXP29FLO8 (aa 206-799) in pGBT9 (BamHI/PstI)This study
pXP38SOK2 in pGAD424 (BamHI/SalI)This study
pXP40PHD1 in pGAD424 (BamHI/PstI)This study
pXP42SFL1 in pGAD424 (BamHI/SalI)This study
pXP70FLO8-GFP in YEplac195This study
pXP94FLO8 in YEplac195This study
pXP96TPK2 (K99R) kinase-inactive allele in pGBT9This study
pXP100Myc12-SWE1 3′UTR in YEplac195This study
pXP101GFP-FLO8 3′UTR in YEplac195This study
pXP110FLO8-MYC12 in YEplac195This study
pXP112SFL1-MYC12 in YEplac195This study
pXP113SFL1-GFP in YEplac195This study
pXP116ADH1-GFP in YCplac33This study
pXP121pADH1-GFP-TPK2 in YCplac33This study
pXP142MBP-FLO8-HIS6 in pMAL2cThis study
pXP143MBP-SFL1-HIS6 in pMAL2cThis study
pXP160pADH1-HA3-SFL1 in YEplac195This study
pXP179TPK2 in YEplac181This study
pXP181SFL1-MYC12 in YEplac181This study
pXP184pFLO8-HA3-FLO8 in YCplac33This study
pXP189FLO8 in YEplac181This study
pXP217FLO8-2 (R155G) in YEplac195This study
pXP223bp −1400 to −1150 region of FLO11 promoter in pCR2.1This study
pXP233bp −2000 to −1750 region of FLO11 promoter in pSLFΔ-178KThis study
pXP234bp −1800 to −1550 region of FLO11 promoter in pSLFΔ-178KThis study
pXP235bp −1600 to −1350 region of FLO11 promoter in pSLFΔ-178KThis study
pXP236bp −1400 to −1150 region of FLO11 promoter in pSLFΔ-178KThis study
pXP237bp −1200 to −950 region of FLO11 promoter in pSLFΔ-178KThis study
pXP238bp −1000 to −750 region of FLO11 promoter in pSLFΔ-178KThis study
pXP239bp −800 to −550 region of FLO11 promoter in pSLFΔ-178KThis study
pXP240bp −600 to −350 region of FLO11 promoter in pSLFΔ-178KThis study
pXP241bp −400 to −150 region of FLO11 promoter in pSLFΔ-178KThis study
pXP242bp −200 to 0 region of FLO11 promoter in pSLFΔ-178KThis study
pXP287pGAL1-GST-TPK2 (K99R) in YEplac195This study
pXP291FLO8-MYC12 in YEplac181This study