TABLE 1.

Yeast strains used in this study

StrainGenotypeReference
Σ1278b strains
    MLY40αMATα ura3-52Lorenz and Heitman (26)
    MLY41aMATa ura3-52Lorenz and Heitman (26)
    MLY42αMATα ura3-52 leu2::hisGLorenz and Heitman (26)
    MLY61a/αMATaura3-52/ura3-52Lorenz and Heitman (26)
    MLY97a/αMATaura3-52/ura3-52 leu2::hisG/leu2::hisGLorenz and Heitman (26)
    MLY162a/αMATapde2Δ::KanMX/pde2Δ::KanMX ura3-52/ura3-52Lorenz and Heitman (26)
    MLY183αMATα tec1Δ::KanMX ura3-52Lorenz and Heitman (25)
    MLY183a/αMATatec1Δ::KanMX/tec1Δ::KanMX ura3-52/ura3-52Lorenz and Heitman (25)
    XPY5αMATα tpk2Δ::KanMX ura3-52Pan and Heitman (35)
    XPY5a/αMATatpk2Δ::KanMX/tpk2Δ::KanMX ura3-52/ura3-52Pan and Heitman (35)
    XPY95αMATα flo8Δ::HygB ura3-52Pan and Heitman (35)
    XPY95a/αMATaflo8Δ::HygB/flo8Δ::HygB ura3-52/ura3-52Pan and Heitman (35)
    XPY108αMATα sfl1Δ::HygB ura3-52This study
    XPY108a/αMATasfl1Δ::HygB/sfl1Δ::HygB ura3-52/ura3-52This study
    XPY116αMATα flo8Δ::HygB sfl1Δ::HygB ura3-52This study
    XPY116a/αMATaflo8Δ::HygB/flo8Δ::HygB sfl1Δ::HygB/sfl1Δ::HygB ura3-52/ura3-52This study
    XPY132αMATα tpk2Δ::KanMX sfl1Δ::HygB ura3-52This study
    XPY132a/αMATatpk2Δ::KanMX/tpk2Δ::KanMX sfl1Δ::HygB/sfl1Δ::HygB ura3-52/ura3-52This study
    XPY133αMATα tec1Δ::KanMX sfl1Δ::HygB ura3-52This study
    XPY133a/αMATatec1Δ::KanMX/tec1Δ::KanMX sfl1Δ::HygB/sfl1Δ::HygB ura3-52/ura3-52This study
    XPY142a/αMATatpk2Δ::KanMX/tpk2Δ::KanMX flo8Δ::HygB/flo8Δ::HygB ura3-52/ura3-52This study
    XPY219a/αMATatpk2Δ::KanMX/tpk2Δ::KanMX ura3-52/ura3-52 leu2::hisG/leu2::hisGThis study
    XPY305a/αMATaflo8Δ::HygB/flo8Δ::HygB ura3-52/ura3-52 leu2::hisG/leu2::hisGThis study
    XPY307a/αMATasfl1Δ::HygB/sfl1Δ::HygB ura3-52/ura3-52 leu2::hisG/leu2::hisGThis study
    XPY308a/αMATaflo8Δ::HygB/flo8Δ::HygB sfl1Δ::HygB/sfl1Δ::HygB ura3-52/ura3-52 leu2::hisG/leu2::hisGThis study
    XPY309a/αMATatpk2Δ::KanMX/tpk2Δ::KanMX sfl1Δ::HygB/sfl1Δ::HygB ura3-52/ura3-52 leu2::hisG/leu2::hisGThis study
Protease-deficient strains
    BJ2168aMATa leu2 trp1 ura3-52 prb1-1122 pep4-3 gal2Jones (20)
    XPY247aMATa leu2 trp1 ura3-52 prb1-1122 pep4-3 gal2 tpk2Δ::KanMXThis study
    BJ5627a/αMATapep4::HIS3/pep4::HIS3 prb1Δ1.6R/prb1Δ1.6R can1/can1 ura3-52/ura3-52 his3Δ200/his3Δ200Jones (20)
    XPY310a/αMATapep4::HIS3/pep4::HIS3 prb1Δ1.6R/prb1Δ1.6R can1/can1 ura3-52/ura3-52 his3Δ200/his3Δ200 GAL1-GST-TPK1/TPK1This study
    XPY311a/αMATapep4::HIS3/pep4::HIS3 prb1Δ1.6R/prb1Δ1.6R can1/can1 ura3-52/ura3-52 his3Δ200/his3Δ200 GAL1-GST-TPK2/TPK2This study
Two-hybrid strains
    PJ69-4AMATa trp1-901 leu2-3,112 ura3-52 his3-200 gal4Δ gal80Δ GAL2-ADE2 LYS2::GAL1-HIS3 met2::GAL7-lacZJames et al. (20)
    XPY100aMATa trp1-901 leu2-3,112 ura3-52 his3-200 gal4Δ gal80Δ GAL2-ADE2 LYS2::GAL1-HIS3 met2::GAL7-lacZ pde2Δ::KanMXThis study
    XPY220aMATa trp1-901 leu2-3,112 ura3-52 his3-200 gal4Δ gal80Δ GAL2-ADE2 LYS2::GAL1-HIS3 met2::GAL7-lacZ tpk2Δ::KanMXThis study