TABLE 3.

The effect of PacC sites on transcription in the wild type and in rim101Δ, rim13Δ, and tup1 mutants

StrainRelevant genotypeβ-Galactosidase activitya for: Repression (n-fold)b
CYC1-lacZCYC1PacC-lacZ
TLY941 RIM101 RIM13 1,5427.3211
WXY281 rim101Δ RIM13 1,7598292.1
WXY278 RIM101 rim13Δ1,0203223.2
AMP620 TUP1 9902.3430
AMP1293 tup1-269 9403222.9
  • a Values are the mean of three or four determinations, and standard deviations were < 25% of the mean.

  • b Values were calculated by dividing the β-galactosidase activity of CYC1-lacZ by that of CYC1PacC-lacZ.