TABLE 1.

ECM alters the entry of T47D cells into mitosisa

TypeHRGSerumEGF
G1SG2/MG1SG2/MG1SG2/M
PL-086.86.56.784.26.98.986.376.7
PL74.415.99.772.115.412.567.815.716.5
LAM66.720.912.463.622.713.77514.910.1
FN75.216.48.468.719.711.675.913.910.2
COL481.77.510.876.99.213.979.49.111.5
POL88.265.880.111.38.6838.98.1
  • a T47D cells were grown on PL, LAM, COL4, FN, or POL, and synchronized as described in the legend to Fig. 1. Cells were left untreated (PL-0) or treated with HRG (20 nM), serum (NuSerum, 10%), or EGF (10 ng/ml) for 18 h and then analyzed by flow cytometry. The percentage of cells in each phase of the cell cycle (G1, S, and G2/M) was measured as indicated.