TABLE 2.

Oligonucleotides used in this study

OligonucleotideSequence
YNL207W-I-NcoI5′-CCCCCCGCCATGGTGAAATTGGATAC TTCTCAT-3′
YNL207W-II-NcoI5′-CCCCCCCCATGGCAGTCGTCTAAACT AAACTA-3′
YNL207W-III-BglIII5′-CCCCCCAGATCTACTAGAAGAAATTG GATACTT-3′
YNL207W-IV-BglIII5′-CCCCCCAGATCTCTAGTATATAGTTTC CTAGTTTATCC-3′
Probe 1 (18S)5′-CATGGCTTAATCTTTGAGAC-3′
Probe 2 (ITS1, D-A2)5′-TTAAGCGCAGGCCCGGCTGG-3′
Probe 3 (ITS1, A2-A3)5′-GATTGCTCGAATGCCCAAAG
Probe 4 (5.8S)5′-TGCGTTCAAAGATTCGATG-3′
Probe 5 (ITS2, A3-C2)5′-GGCCAGCAATTTCAAGTTA-3′
Probe 6 (25S)5′-CTCACGACGGTCTAAACCC-3′
Probe 7 (ETS1, A0-A1)5′-CACCCATTCCCTCTTGCTAG-3′