TABLE 1.

pVHL can associate with translation regulatory factors in yeasta

Candidate pVHL-interacting proteinsGenBank accession no.FunctionNo. of clones
Translation initiation factor 3 subunit 4 (eIF3)NP003746Translational regulation1
Translation elongation factor 1-alpha (EF-1α) (EF-TU)AAA52367Translational regulation4
Glucose-regulated protein (GRP 78)H56456S1Molecular chaperone1
Heat shock 60-kDa protein 1 (HSP 60)P10809Molecular chaperone1
Heat shock protein 90 (HSP 90)HHHU86Molecular chaperone2
VHL-binding protein 1 (VBP-1)Q15765Cytoplasmic export of pVHL1
26S protease regulatory subunit (Tat-binding protein 1)P17980Protein degradation1
GelsolinNP000168Other1
Metallothionein 2AH56373S3Other1
Filamin (actin-binding protein 280) ABP-280NM001456Other1
RIE 2 sid 2705BAA84708Other1
CGI-111 proteinAAD34106Other1
Antioxidant enzyme BP 66AAF03750Other1
Gene SA proteinI54401Other1
X-Pro dipeptidaseL10320Other2
  • a Shown are the results of a yeast two-hybrid survey leading to the identification of potential pVHL-interacting proteins from a human kidney cDNA library. Some of the clones were represented several times (number of clones, right column). The interaction of pVHL with all of the gene products listed was confirmed by yeast mating (explained in Materials and Methods).