TABLE 4.

Northern blot analysis of cofactor mutants

Relevant genotypeCofactor complexMean normalized signal ratio (% of WT)a
SNZ1, inducedHIS4ILV2ARG1
UninducedInducedUninducedInducedUninducedInduced
gcn4ΔActivator12b,c67b,c31b,c78c32c51b,c14b,c
mbf1ΔNAd63c73c68c77c
ada2ΔADA, SAGA70b47b43b207b96b
gcn5ΔADA, SAGA124b,c105b,c98b,c38c71c313b,c143b,c
ada1ΔSAGA47c90c29c69c59c915c101c
ada5ΔSAGA45c27c45c106c
spt3ΔSAGA68b69b52b130b86b
spt7ΔSAGA41b458b121b
swi2ΔSWI/SNF44b,c139b206b109c118b,c
swp73ΔSWI/SNF30b32b
rsc1ΔRSC67c118c88c88c70b,c198c67c
rsc2ΔRSC144b,c242c63c99c124c>600c111c
not5ΔCCR4-NOT155c118c74c85c79c91c72c
caf1ΔCCR4-NOT76b,c103c77c51c62c71c48c
dhh1ΔCCR4-NOT65b,c135b,c102b,c96c56c99c41c
srb5ΔSRB/MED126b,c307c118c152c92c>300b,c148b,c
pgd1ΔSRB/MED62c144b,c67b,c114c72c474c57c
rox3ΔSRB/MED38b,c129b,c67b,c83c62c105b,c52b,c
sin4ΔSRB/MED, Paf168c125c94c91c86c379c85c
gal11ΔSRB/MED, Paf155c78b,c102b,c118c78c213b,c97b,c
med2ΔSRB/MED65c76c84c168c88c171c83c
cdc73ΔPaf1 complex98c84c77c100c
ccr4ΔCCR4-NOT, Paf157b,c70b,c49b,c60c51c70c44c
hpr1ΔPaf1, THO/TREX49b,c59b,c50b,c54c125c65c66c
gcn4Δ/gcn4ΔActivator16c57c23c81c26c54c7c
paf1Δ/paf1ΔPaf1 complex97c327c126c120c136c2,770c149c
snf5Δ/snf5ΔSWI/SNF75c93c85c261c162c500c104c
  • a For each value shown, at least two independent cultures of each strain were grown under inducing or noninducing conditions (presence or absence of SM) as described in Materials and Methods, and equal amounts of isolated total RNA were subjected to Northern analysis. The hybridization signals for SNZ1, HIS4, ILV2, and ARG1 were normalized to corresponding ACT1 signals. The resulting ratios calculated for the mutant strains were normalized to the ratio measured for the WT, and the mean normalized ratios are shown as a percentage of the WT value. The mean values were calculated from results that yielded standard errors of 30% or less. Results in italics are 200% of the WT value or greater. Underlined results are <68% of the WT value under inducing conditions and show reductions relative to WT that are ∼2-fold (bold face type with an asterisk), 1.5-fold (bold face type), or less than 1.5-fold greater under inducing than noninducing conditions.

  • b Induction as per the ChIP assay.

  • c Induction as per the reporter assay.

  • d NA, not applicable.