TABLE 1.

Fission yeast strains used in this study

StrainGenotype
JY333h ade6-M216 leu1
JY450h90 ade6-M216 leu1
JZ804h+ mei4::ura4+ leu1 ura4-D18 ade6-M210
PY182h90 rec8::kanr ade6-M216 leu1
PY221h+/h+ pat1-114/pat1-114 cdc2-L7/cdc2-L7 rec8+-GFP-kanr/rec8+-GFP-kanr ade6-M210/ade6-M216 leu2/+ +/lys1
PY322h+ rec8::kanr lys1+<<lacO his7+<<Pdis1-GFP-lac1-NLS leu 1 ade6-M216
PY445h90 mei4::ura4+ rec8::kanr rad21-K1<<ura4+ lys1+<<lacO his7+<<Pdis1-GFP-lac1-NLS leu1 ade6-M216
PY446h90 mei4::ura4+ rec8::kanr lys1+<<lacO his7+<<Pdis1-GFP-lac1-NLS leu1 ade6-M216
PY447h90 mei4::ura4+ lys1+<<lacO his7+<<Pdis1-GFP-lacI-NLS leu1 ade6-M216
PY448h90 mei4::ura4+ rad21-K1<<ura4+ lys1+<<lacO his7+<<Pdis1-GFP-lac1-NLS leu1 ade6-M216
PY545h rec8::kanr leu1
PY594h90 mei4::ura4+ ade3::lacO-ura4+-kanr his7+<<Pdis1-GFP-lacI-NLS leu1 lys1 ade6
PY595h90 mei4::ura4+ ade3::lacO-ura4+-kanr his7+<<Pdis1-GFP-lacI-NLS leu1 lys1 ade6
PY741h+/h mei4::ura4+/mei4::ura4+ rec8+-GFP-kanr/rec8+-GFP-kanr ade6-M216/ade6-M210
PY748h rec8Δ::Padh1-rad21+-FLAG ade6-M210
PY749h rec8Δ::Prec8-rad21+-FLAG ade6-M210
PY754h+/h+ pat1-114/pat1-114 cdc2-L7/cdc2-L7 rec8Δ::Padh1-rad21+-GFP/rec8Δ::Padh1-rad21+-GFP leu2/+ +/lys1 ade6-M216/ade6-M210
PY770h+/h+ pat1-114/pat1-114 cdc2-L7/cdc2-L7 rec8Δ::Prec8-rad21+-GFP/rec8Δ::Prec8-rad21+-GFP leu2/+ +/lys1 ade6-M216/ade6-M210
PY783h rad21-K1<<ura4+ rec8::kanr leu1 ade6-M216
PY785h+ rad21-K1<<ura4+ rec8::kanr lys1+<<lacO his7+<<Pdis1-GFP-lacI-NLS ade6-M216 leu1
PY786h rad21-K1<<ura4+ ade6-M216
PY787h+ rad21+-GFP-kanr mei4::ura4+ leu1 ura4-D18 ade6-M210
PY801h90 rad21+-GFP-kanr leu1 ade6-M210
PY878h90 rec8::ura4+ rad21+-GFP-kanr ade6-M216
PY893h+/h+ pat1-114/pat1-114 cdc2-L7/cdc2-L7 rec8::kanr/rec8::kanr rad21+-GFP-kanr/rad21+-GFP-kanr leu2/+ +/lys1 ade6-M216/ade6-M210
PZ207h90 rec8Δ::Padh1-rec8+-3HA lys1+<<lacO his7+<<Pdis1-GFP-lacI-NLS leu1 ura4-D18 ade6
PZ208h90 rec8Δ::Padh1-rec8+-3HA rad21::ura4+ lysl+<<lacO his7+<<Pdis1-GFP-lac1-NLS leu1 ura4-D18 ade6