TABLE 1.

In vitro colony formation capacity of mutant PIM and wild-type bone marrow cells in response to distinct growth factors

Growth factorColony typeMean (±SD) colony formation capacity (n) for mice of Genotypea
Pim1+/+2+/+3+/+Pim1−/−2+/+3+/+Pim1+/+2−/−3+/+Pim1+/+2+/+3−/−Pim1−/−2−/−3−/−
IL-3CFU-mix382 ± 43 (16)194 ± 53b (3)381 ± 14 (2)336 ± 55 (6)120 ± 37b (12)
SCFCFU-mix119 ± 10 (16)82 ± 20 (3)126 ± 5 (2)95 ± 21b (5)64 ± 12b (12)
GM-CSFCFU-GM358 ± 32 (18)402 ± 62 (2)365 ± 30 (2)385 ± 43 (3)357 ± 42 (12)
TPOCFU-Meg17.9 ± 2.5 (9)0.26 ± 0.31 (3)19.6 ± 0.8 (2)18 ± 3 (6)0 ± 0b (9)
IL-5CFU-Eos19.6 ± 2.1 (10)8 ± 5.7 (2)20.4 ± 0.4 (2)18.6 ± 6.3 (3)2 ± 1b (9)
  • a Values represent numbers of colonies. n, number of mice. Pim1+/+2+/+3+/+, Pim1+/+ Pim2+/+ Pim3+/+.

  • b P < 0.05 (t test analysis compared to the wild type).