TABLE 4.

Mitotic and meiotic levels of hopI-lacZ expression

Strainaβ-Galactosidase activity (Miller units)
SD SA SPM
25°C34°C25°C34°C25°C34°C
Wild type0.100.040.978.98435.68948.05
cdc25-5 0.3912.238.85129.14599.15422.40
rim11Δ0.060.420.061.570.020.30
rim11K68A NTNT0.010.450.020.03
rim11Δcdc25-5 0.050.140.9811.110.020.36
  • a The strains used were diploids constructed by the following matings: wild type, Y1064 × Y1065; cdc25-5, Y1078 × Y1120; rim11Δ, Y1066 × Y1089; rim11Δ cdc25-5, Y1084 × Y1093; and rim11K68A, Y1174 × Y1089. These cells carry the hop1-lacZ chimera on a 2μm TRP1 vector (YEp2042). Cells grown at 25°C to 0.5 × 107 cells/ml were shifted to 34°C for 6 h. Cells grown at 25°C in SA were shifted to SPM and incubated at 25 or 34°C for 6 h. NT, not tested.