TABLE 2.

Plasmids used in this study

PlasmidCharacteristicsSource or reference
pAS55YEplac195::HA-TAT23
pAS103YEplac195::HA-NPR154
pTB374pHAC195::HXT1 (2μm URA3) (C-terminally 3× HA tagged, CYC1 terminator; 2.2-kb XbaI fragment)This study
pTB380pHAC111::HXT1 (CEN LEU2) (C-terminally 3× HA tagged, CYC1 terminator; 2.2-kb XbaI fragment)This study
pTB419pHAC195::BMH2 (2μm URA3) (C-terminally 3× HA tagged, CYC1 terminator; 1.5-kb BamHI-PstI fragment)This study
pTB424YEplac195::CDC25ΔN (2μm URA3) (CDC25 promoter, 3× HA CDC25ΔN; 0.9-kb BamHI-XbaI [promoter] and 1.6-kb XbaI [3× HA-CDC25 ORF C terminus including GEF domain/terminator] fragments)This study
pTB429YEplac195::SOK1 (2μm URA3) (3.7-kb BamHI-PstI fragment)This study
pTB439YEplac195::TPK1 (2μm URA3) (2.2-kb BamHI-HindIII fragment)This study
pTS118YCplac33::RAS2Val19 (CEN URA3) (2.1-kb EcoRI-HindIII fragment)This study
pTS119YCplac33::RAS2Ala18Val19 (CEN URA3) (2.1-kb EcoRI-HindIII fragment)This study
pTS120YCplac111::RAS2Val19 (CEN LEU2) (2.1-kb EcoRI-HindIII fragment)This study
pTS134YCplac33::HA-TPK1 (CEN URA3) (N-terminally 2× HA tagged; 0.7-kb BamHI-PstI [promoter] and 1.5-kb PstI-HindIII [2× HA TPK1 ORF/terminator] fragments/2.2-kb EcoRI-HindIII fragment)This study
pTS137YCplac33::HA-BCY1 (CEN URA3) (N-terminally HA -tagged; 0.3-kb EcoRI-BamHI [promoter + atg] and 1.6-kb BamHI-Hind III [HA-BCY1 ORF/terminator] fragments)This study
pG2CT-112.2GPA2R273A expressed under control of GAPDH promoter (2μm TRP1)66
pMSN2-GFPYCplac111::ADH1 promoter-MSN2-GFP20
pTN9TDH3 promoter-pho8Δ6045