TABLE 1.

Steady-state changes in gene expressiona

Category and probe setFold changePoly 16/0GenBank no.DescriptionReference(s)
Energy generation and metabolism
    40193_at1912.9X51956ENO2 gene for neuron specific (gamma) enolase24
    37188_at1412.7X92720Phosphoenolpyruvate carboxykinase42
    1586_at1118.8M35878Human insulin-like growth factor-binding protein 313
    32331_at67.4X60673Adenylate kinase 3 (AK3)61
    31488_s_at52.3S81916Phosphoglycerate kinase (alternatively spliced)24
    40107_at35.5AF054987Aldolase C24
    38042_at2.41.6X03674Glucose-6-phosphate dehydrogenase (G6PD)
    36979_at2.43.1M20681Glucose transporter-like protein III (GLUT3)24, 61
    35938_at−12−10.0M72393Calcium-dependent phospholipid-binding protein (PLA2)
    262_at−3.8−3.6M21154S-Adenosylmethionine decarboxylase
Angiogenesis or vessel dynamics
    1103_at9.215.0M11567Angiogenin20
    34777_at8.717.4D14874Adrenomedultin precursor10
    36606_at5.92.9X51405Carboxypeptidase E
    36100_at45.4AF022375Vascular endothelial growth factor (VEGF)7, 24, 50
Matrix metabolism and pH regulation
    40309_at8385.6X66839Carbonic anhydrase IX37, 63
    926_at208.8J03910Metallothionein-IG (MTIG)
    34390_at2020.4U90441Human prolyl 4-hydroxylase alpha (II) subunit60
    38637_at17.320.5L16895Human lysyl oxidase (LOX)
    33127_at13.511.9U89942Human lysyl oxidase-related protein (WS9-14)
    37037_at10.214.9M24486Human prolyl 4-hydroxylase alpha subunit60
    41750_at8.18.6D49489Protein disulfide isomerase-related protein P5
    34795_at7.85.4U84573Lysyl hydroxylase isoform 2 (PLOD2) mRNA
    36454_at5.89.6AF037335Carbonic anhydrase precursor (CA12)63
    36666_at5.75.1M22806Prolyl 4-hydroxylase β-subunit60
Cell stress response or DNA repair
    442_at12.215.8X15187tra1 mRNA
    36614_at11.39.4X87949BiP36
    38986_at6.37.2Z49835Protein disulfide isomerase (glucose-related protein 58)
    1955_s_at−6.1−10.6AF035528Smad6 mRNA
    1104_s_at−5−3.8M11717Human heat shock protein (hsp 70)
    36785_at−3.3−2.5Z2309028-kDa heat shock protein
Cell death and proliferation
    37168_at16.520.7AB013924TSC403 protein
    39436_at15.18.3AF079221BCL2/adenovirus E1B 19-kDa interacting protein 3a
    654_at15.312.5L07648Human MXI1 mRNA
    2011_s_at11.93.8U34584Human Bcl-2 interacting killer (BIK)27
    38010_at10.810.4AF002697Homo sapiens E1B 19K/Bcl-2-binding protein Nip36
    37028_at3.46.0U83981Apoptosis-associated protein (GADD34)34
    37228_at−4.6−5.9U01038pLK mRNA
Cell cycle
    1913_at3.52.9U47414Cyclin G2
    1206_at−7.3−2.3X66364PSSALRE for serine/threonine protein kinase
    38414_at−5.3−3.6U05340p55CDC
    1945_at−4.7−7.0M25753Cyclin B
    1794_at−3.9−3.9M92287Cyclin D3 (CCND3)12
    1943_at−3.3−2.4X51688Cyclin A
    35699_at−3−4.4AF053306Mitotic checkpoint kinase Mad3L
    1803_at−2.3−2.7X05360CDC2
RNA splicing or transcription
    36618_g_at−23−12.0X779561d1 mRNA50
    351_f_at−4−2.4D28423Pre-mRNA splicing factor SRp20
  • a Results of Affymetrix HGU95A-V2 microarray analysis used to identify steady-state changes in gene expression during hypoxia. Affymetrix MAS5.0 software and Genespring 5.0 were used to compare 16-h total to 0-h total RNA to determine gene changes induced or repressed >2-fold. Genes were functionally clustered and referenced if previously identified in the literature. Only named genes are included in this list; expressed sequence tags were omitted. Analysis is representative of two independent microarray analyses performed with RNA isolated from two independent experiments. Values for decreased candidates are in boldface.