TABLE 1.

Effect of eRF3 mutations on translation termination

Stop signalb% Readthrough fora:Change (fold) (relative to wild type) forc:
Wild typeR419GH348QR419GH348Q
UAAA0.30 ± 0.010.27 ± 0.050.27 ± 0.070.90.9
UAAC0.45 ± 0.041.95 ± 0.151.52 ± 0.284.43.4
UAAG0.30 ± 0.010.27 ± 0.040.36 ± 0.060.91.2
UAAU0.25 ± 0.020.50 ± 0.050.24 ± 0.022.00.9
UAGA0.16 ± 0.030.24 ± 0.030.26 ± 0.031.51.7
UAGC0.33 ± 0.021.31 ± 0.171.03 ± 0.154.03.2
UAGG0.26 ± 0.010.25 ± 0.010.24 ± 0.031.00.9
UAGU0.12 ± 0.010.12 ± 0.010.14 ± 0.021.11.2
UGAA0.49 ± 0.062.42 ± 0.102.90 ± 0.184.95.9
UGAC1.40 ± 0.4011.6 ± 1.0023.9 ± 1.808.216.8
UGAG0.85 ± 0.143.10 ± 1.003.90 ± 0.423.74.6
UGAU0.21 ± 0.031.10 ± 0.20.94 ± 0.065.24.5
  • a Percent readthrough is expressed as the mean ± standard deviation.

  • b All measurements were carried out with the [psi] strain YDB498 (sup35Δ) carrying pDB663 (wild-type eRF3), pDB783 (eRF3-R419G), or pDB670 (eRF3-H348Q).

  • c Changes in relative readthrough >2-fold are underlined.