TABLE 3.

Effect of eRF3 depletion on translation termination

Stop signalb% Readthrough fora:Change (fold)
PSUP35PCUP1
UAAA0.16 ± 0.020.78 ± 0.025.0
UAAC0.15 ± 0.011.2 ± 0.18.2
UAAG0.13 ± 0.020.71 ± 0.125.6
UAAU0.08 ± 0.010.79 ± 0.079.6
UAGA0.10 ± 0.020.64 ± 0.16.4
UAGC0.20 ± 0.031.7 ± 0.178.3
UAGG0.13 ± 0.031.0 ± 0.158.1
UAGU0.06 ± 0.010.30 ± 0.035.4
UGAA0.17 ± 0.011.3 ± 0.147.7
UGAC0.35 ± 0.015.3 ± 0.515.1
UGAG0.11 ± 0.021.8 ± 0.0616.4
UGAU0.07 ± 0.020.96 ± 0.213.3
  • a Percent readthrough is expressed as the mean ± standard deviation.

  • b All measurements were carried out using the [psi] strains 614 (wild type) or YDB498 (sup35Δ) carrying pDB824, which expresses eRF3 from the CUP1 promoter.