TABLE 2.

Effect of eRF1 depletion on translation termination

Stop signalb% Readthrough fora:Change (fold)
PSUP45PCUP1
UAAA0.19 ± 0.020.94 ± 0.055
UAAC0.37 ± 0.062.8 ± 0.277.5
UAAG0.22 ± 0.021.2 ± 0.075.3
UAAU0.28 ± 0.010.97 ± 0.103.4
UAGA0.18 ± 0.010.60 ± 0.053.3
UAGC0.49 ± 0.022.0 ± 0.184.1
UAGG0.13 ± 0.010.80 ± 0.046.5
UAGU0.12 ± 0.020.70 ± 0.075.9
UGAA0.37 ± 0.044.6 ± 0.5012.5
UGAC1.3 ± 0.1211.7 ± 0.959
UGAG0.55 ± 0.031.9 ± 0.233.5
UGAU0.24 ± 0.051.2 ± 0.25
  • a Percent readthrough is expressed as the mean ± standard deviation.

  • b All measurements were carried out using the [psi] strains YDB340 (wild type) or YDB447 (sup45Δ) carrying pDB811, which expresses eRF1 from the CUP1 promoter.