TABLE 2.

Hypoxia-induced translationally regulated genesa

FunctionPoly 16/0ANOVA valueTotal 16/0GenBank no.Name: derivation
Antiapoptosis or apoptosis4.10.1792.2U33821TXBP151: Tax1-binding protein
2.70.2431.3X75861TEGT: testis enhanced gene transcript (BAX inhibitor 1)
2.70.0151.8S81914IER3: immediate early response 3
2.30.1001.5Z15108PRKCZ: protein kinase C, zeta
2.30.0071.9AB020680BAG5: BCL2-associated athanogene 5
2.00.0631.8AF099935GG2-1: tumor necrosis factor-induced protein
Cell cycle regulator2.00.0401.8U22398KIP2: Cdk-inhibitor p57KIP2
DNA repair2.40.1261.7D83702CRY1: cryptochrome 1
2.30.1131.4D21089XPC: xeroderma pigmentosum
Metabolism5.00.0231.2AF061741SDR1: short-chain dehydrogenase/reductase 1
3.50.0521.6AA873266PDK3: pyruvate dehydrogenase kinase
2.90.1481.9Y10275PHKG1: phosphorylase kinase
2.10.2051.2X69433IDH2: isocitrate dehydrogenase 2
1.90.0641.1M15182GUSB: glucuronidase
1.60.2881.6X15573PFKL: phosphofructokinase
Stress response/UPR9.00.0132.7L19871ATF3: Activating transcription factor 3
5.00.0371.2AL022312ATF4: activating transcription factor 4
2.40.1291.7D83702Casein kinase 1, epsilon
2.00.3361.8AB017642OSR1: oxidative-stress responsive 1
2.00.2501.0X68277DUSP1: dual specificity phosphatase 1
1.90.0540.9AF005887ATF6: activating transcription factor 6
1.90.0801.0U09759MAPK9: mitogen-activated protein kinase 9
Regulation of transcription3.10.2572.5U49020MEF2A: MADS box transcription enhancer factor 2
3.00.1810.5AB015051DAXX: death-associated protein 6
2.80.0101.3AB000468RNF4: ring finger protein 4
2.60.0331.9U11861G10: maternal G10 transcript
2.50.1681.0AF069733TADA3L: transcriptional adaptor 3
2.30.0172.0X51345JUNB: junB proto-oncogene
2.30.1131.6M95929PMX1: paired mesoderm homeobox
2.20.2161.5X91648PURA: purine-rich element binding protein A
2.20.0681.9AF038177FHX: FOXJ2 forkhead factor
2.10.0221.4M34539FKBP1A: FK506 binding protein 1A
2.10.5001.1M25077SSA2: Sjögren syndrome antigen A2
2.00.0251.2Z35278RUNX3: runt-related transcription factor 3
2.00.0271.1AF078096FOXC1: forkhead box C1
2.00.0041.6U71300SNAPC3: small nuclear RNA activating complex
1.90.0970.9M16801NR3C2: nuclear receptor
1.80.4801.9M27691CREB1: cAMP responsive element binding protein 1
1.80.7021.5AF019214HBP1: HMG-box containing protein 1
Signal transduction4.00.0701.4L11284MAP2K1: mitogen-activated protein kinase kinase 1
2.20.1671.6M62403IGFBP4: insulin-like growth factor binding protein 4
2.00.1541.3M97815CRABP2: cellular retinoic acid binding protein 2
Protein biosynthesis2.80.0041.7X94754MARS: methionine-tRNA synthetase
2.50.0142.0AL050281NAG: neuroblastoma-amplified protein
Regulation of translation3.40.4591.7U49436eIF5: eukaryotic translation initiation factor 5
Angiogenesis2.20.0321.3U43368VEGFB: vascular endothelial growth factor B
2.00.0530.7M27968FGF2: fibroblast growth factor 2
Iron ion transport5.50.0001.3L20941FTH1: ferritin, heavy polypeptide 1
Proteolysis3.50.0481.9AB029027PITRM1: pitrilysin metalloproteinase 1
  • a Results of microarray analysis to identify transcripts that were specifically associated with high-molecular-weight polysomes in hypoxia-treated cells. Genespring 5.0 software was used to compare the relative expressions of polysome-bound mRNAs in hypoxic (16-h poly) and normoxic (0-h poly) cells. Genes that were induced >2-fold in the polysomes (poly 16/0) and induced <3-fold in the total cellular mRNA (total 16/0) were considered for further analysis. ANOVA was used to assess the statistical significance of the changes in mRNA found in polysomal fractions. In this analysis, reported P values between 0 and 0.1 represent highly significant changes with little variance in the data set. P values between 0.1 and 0.3 represent changes that have a higher degree of variation between replicate experiments but are maintained as biologically relevant changes. There is a high probability that differences observed between hypoxic and normoxic samples are due to chance when ANOVA P values exceed 0.5. A subset of the genes identified by this analysis were functionally clustered and are represented in this table.