TABLE 1.

Sir1p amino acid changes

Amino acid change(s)aOrc1p or Sir4p interaction defect(s)
E486-488ANeither
V490G*Orc1p
R493G*bOrc1p
F494S*Orc1p and Sir4p
L501P*Orc1p and Sir4p
D503N*Orc1p
L504P*Orc1p
E506-507ANeither
K513A, D514ANeither
K522A, D523ANeither
W537R*Orc1p and Sir4p
K541-542ANeither
C558ANeither
K562-564ASir4p
C595R*Orc1p and Sir4p
V596GNeither
P597SNeither
D601-602ASir4p
L608P*Orc1p and Sir4p
L608Q*Orc1p and Sir4p
D610-611ASir4p
  • a *, mutants that were generated through random mutagenesis. All other mutants were generated using site-directed mutagenesis.

  • b This mutation was isolated and described in an earlier study (14).