TABLE 1.

Inhibitors used to screen for protein kinase-dependent pS-289 phosphorylation

InhibitorConcn (μM)Targeta
LY29400225PI3K
Go69760.02, 1, 10PKCα, PKCβ1
Go685010, 20PKC including ζ
H9 dihydrochloride1, 10PKA
H9 dihydrochloride200PKC
KT57200.3, 1, 10PKA
KT58231, 2PKG
Y2763210Rok
PD9805950Mek
Apigenin20, 80CK2
KN931, 10, 30CAM kinase II
W71, 10, 30CaMK, MLCK
  • a Abbreviations: PI3K, PI 3-kinase; CAM, calmodulin-dependent; CaMK, calmodulin-dependent kinase; MLCK, myosin light-chain kinase.